响应面法优化提取花椒籽蛋白质工艺研究

本研究以花椒籽为原料,采用盐提法提取花椒籽中蛋白质。在单因素实验基础上,根据Central-Composite中心组合实验设计原理,采用响应面分析法,考察NaCl浓度、提取时间、提取温度、料液比、pH值对蛋白质得率的影响。结果表明,响应面法优化所得的最佳工艺条件可靠,其最佳工艺条件为:NaCl浓度0.79mol·L-1,提取时间60min,提取温度35℃,料液比1∶17.5,pH值8.78。在此最佳工艺条件下花椒籽粗蛋白得率为18.92%。     

Characteristics of soils derived from tephra and pyroclastic flow deposits from Taal volcano,the Philippines

The various morphological, physico-chemical and mineralogical properties of soils derived from tephra and pyroclastic flow deposits of the Taal volcano were characterized and evaluated.

1. As compared with the pumiceous sandy soils of Mt. Pinatubo, soils of the Taal volcano showed generally a finer texture (silty clay to clay) with the exception of the base surge materials consisting of loam.

2. Parent ashes ranged from basaltic-andesite to basalt, as compared with andesitic to dacite for Mt. Pinatubo. Lithologic nature as well as the age of the tephra seemed to account for the advanced degree of weathering and resultant high clay content in the Taal soil.

3. Clay consisted predominantly of crystalline silicate mineral dominated by halloysite. Dominance of crystalline clay could be due to the ustic moisture conditions with a long dry period. In common with Mt. Pinatubo soils, soils of the flood plains were dominated by 2 : 1 clay minerals.

4. Based on the data on andic soil properties the soils studied could not be classified as Andisols. Basically, the typical andic soil properties did not develop under a ustic soil moisture regime which contributed to the clay mineral composition. Two of the pedons examined in this study were classified as Andic Eutropepts while on the pedons studied had been classified as Ustarent.     

A new method for measuring microbial biomass nitrogen in soils: Direct extraction after toluene treatment

Abstract

Phenolic compounds occurring naturally are reducing agents which react with hydrous Fe oxide and Mn oxides (Lehmann et al. 1987). Diphenols such as hydroquinone, resorcinol, and catechol were oxidatively darkened by the presence of hydrous Fe oxide though the degree of darkening was much less pronounced than that by Mn oxides (Shindo and Huang 1984). Okazaki et al. (1976) suggested that polyphenols are one of the materials responsible for the dissolution of Mn in a paddy soil under reducing conditions.     

Role of active aluminum in the formation of water-stable macroaggregates

Soil structure is determined by the arrangement of particles in soil and the particles of sand, silt, and clay bind together into aggregates of various sizes by organic and inorganic materials. Structural stability which is the ability of the aggregates and pores to remain intact when subjected to stress, markedly affects crop production and soil erosion (Tisdall 1996). Since water, either directly as rainfall or as surface runoff is the main agent of aggregate breakdown, in the analyzes of stable soil aggregation, the term water-stable aggregation is generally used (Lynch and Bragg 1985). Water-stable aggregates have been divided into micro aggregates < 0.25 mm dia.) and macro aggregates (> 0.25 mm dia.) (Edwards and Bremner 1967; Tisdall and Oades 1982). Microaggregates show a relatively high stability against physical disruption (Edwards and Bremner 1967). On the other hand, macro aggregates are sensitive to soil management (Tisdall and Oades 1982).

There are many reports on the relationships between the aggregate stability and the soil physicochemical properties. For example, significant correlations were found between the aggregate stability and the amounts of organic C (Tisdall and Oades 1982), total N, and carbohydrates or the CEC (Chaney and Swift 1984). However, most of these studies were conducted in non-volcanic ash soils. Volcanic ash soils are widely distributed in Japan and are very important soils for crop production. The objective of this study was, therefore, to obtain more information on the relationship between the degree of macro aggregation and the soil physicochemical properties in non-volcanic and volcanic ash soils.     

Combining pre-transplanting phosphorus application and green manure incorporation: a trial for reducing fertilizer input

Pre-transplanting phosphorus application is a fertilization method that can reduce phosphorus (P) fertilization of the field and increase P use efficiency. We investigated whether P use efficiency can be increased further by combining pre-transplanting phosphorus application and the green manure practice, using cabbage as a test plant in a field experiment performed from 2006 to 2008. The pre-transplanting phosphorus application successfully reduced P fertilization of the field to half without any yield losses in 2006 and 2008. However, growth and yield could not be maintained with the pre-transplanting phosphorus application when P fertilization was halved in 2007. Differences among seasons and discrepancies in the results compared to previous studies may have arisen from the differences in the field management and the nursery soil used. No benefits of green manure in terms of the subsequent cabbage yield were detected. Consequently, there was no positive effect of the combination of pre-transplanting phosphorus application and incorporation of green manure. Studies with mycorrhizal plants as test plants may be necessary to verify the mechanism of enhanced P supply by the incorporation of green manure, and to examine any potential benefit of combining green manure and pre-transplanting phosphorus application practices.     

供暖锅炉消烟除尘装置的选择

各种锅炉消烟除尘设备都有自己的使用范围,本文主要介绍了供暖锅炉在消烟除尘选用中应考虑的问题,阐明选用消烟除尘设备时要充分了解其技术范围及其经济性能。     

加热炉管外积灰及减少积灰的措施

分析了输油管道直接式加热炉炉管积灰的形成、灰垢的种类和成分。认为灰垢就是燃料油燃烧后残留下来的不可燃部分Ｎａ、Ｋ、Ｖ、Ｍｇ等金属固体盐类和其它杂质，以及碳元素在燃烧不完全的情况下残留下的可燃组分的微粒。指出燃料灰分含量的高低、燃烧器雾化质量好坏、燃烧空气温度的高低及操作人员的技术水平的高低都是影响灰垢生成量的主要因素。分析了辐射室炉管外壁和对流炉管外壁的积灰规律。指出管外积灰增加了热阻，影响传热效     

Foliar residue dynamics of azadirachtins following direct stem injection into white and green ash trees for control of emerald ash borer

BACKGROUND: Azadirachtins are natural insecticides derived from the neem tree. The emerald ash borer (EAB) is an exotic invasive insect pest that infests various ash tree species and has the potential for significant economic, aesthetic and ecological impacts throughout North America. The initial translocation and foliar residue dynamics of azadirachtins were examined following direct injection into white and green ash trees growing in urban scenarios as a potential control for EAB. RESULTS: Substantial concentrations of azadirachtins A and B [mean maxima > 0.98 mg kg^?1 fresh weight (f.w.)] were observed within 2 days of injecting a specifically designed formulation of azadirachtins. Foliar residues declined exponentially through time, with half‐life estimates ranging from 5.1 to 12.3 days. At the time of leaf senescence, foliar residue levels approximated 0.01 mg kg^?1 f.w., strongly mitigating the potential effects of non‐target biota in soil or aquatic compartments. CONCLUSION: The magnitude and duration of exposures observed in this field study were considered to be above the thresholds required for biological effectiveness against both larval and adult life stages of EAB. Results support the use of azadirachtins as an environmentally acceptable systemic insecticide for control of EAB and protection of high‐value ash trees in urban environments. Copyright © 2011 Society of Chemical Industry     

成型和灼烧温度对垃圾衍生燃料灰渣理化特性的影响

以生活垃圾衍生燃料(refuse derived fuel,RDF)为研究对象,通过挤压成型的RDF与非成型的RDF在600~1 100℃条件下灼烧,所产生的灰渣进行质量、粒径、化学成分等特性进行分析,探索RDF灰渣随温度变化的规律以及潜在的应用价值。研究结果表明,在1 000℃以下,RDF灰渣随着温度提高,灰分组分发生分解反应,主要是残余有机物、碳酸盐、硫酸盐的分解,灰渣粒径减小;当温度升至1 000℃以上,灰分组分发生化合反应,生成产物以复合硅酸盐为主,灰渣粒径增大甚至结块;灰渣在化学反应过程中,非金属元素C,Cl,S转化为气体化合物逸出主体,使灰渣质量减小;成型RDF灰渣晶体含量比非成型RDF高;成型RDF灰渣粒径比非成型RDF灰渣大。     

锯木灰渣改良退化菜园紫色土壤的效果研究

以重庆市九龙坡区白市驿镇蔬菜基地为试验平台,通过大田试验,研究了锯木灰渣不同施用量还田1年后对酸性紫色土改良效果,并进一步分析了锯木灰渣对土壤微生物群落及微生物碳、氮的影响,以期为紫色菜园退化土壤养分和酸化的修复提供合理措施,同时实现循环农业的可持续发展。结果表明:(1)锯木灰渣施用能显著提高蔬菜产量,且随着施用量的增加呈增加趋势,其中锯木灰渣施用量为18 000 kg hm~(-2)时,产量较未施用锯木灰渣(A0)处理显著提高了76.1%~121.9%;(2)经锯木灰渣处理的退化紫色土表层和亚表层的有机质含量和pH提高,尤其是锯木灰渣施用量为18 000 kg hm~(-2)的改良效果较佳,但是过高的锯木灰渣施用量处理对土壤交换性H~+、Al~(3+)效果反而下降,而施用量为12 000~15 000 kg hm~(-2)处理的效果较佳,其中表层土壤交换性H~+、Al~(3+)分别为0.19~0.20,0.63~0.67 cmol kg~(-1);(3)12 000~15 000 kg hm~(-2)的锯木灰渣施用量对土壤碱解氮和速效钾的提升效果较佳,18 000 kg hm~(-2)处理的有效磷含量最大,较A0处理增加了55.3 mg kg~(-1);(4)无论在表层土壤还是亚表层土壤中,细菌的种群数量分布最多,其次为真菌和放线菌,分别在12 000,9 000,6 000 kg hm~(-2)锯木灰渣施用量下的种群数量达到最大值;9 000~12 000 kg hm~(-2)处理对提高表层土壤微生物量碳含量较为明显,较A0显著提高了70.4%~78.7%,18 000 kg hm~(-2)处理的土壤微生物生物量氮(SMBN)含量最大(40.54 mg kg~(-1)),其次为9 000 kg hm~(-2)处理(34.32 mg kg~(-1))。综上,锯木灰渣施用量为12 000~15 000 kg hm~(-2)时对退化紫色土的改良效果较好。